大肠杆菌中的新型极性抑制子：抑制作用与信使核糖核酸稳定性
New polarity suppressors in Escherichia coli: suppression and messenger RNA stability.
作者信息
Carter T, Newton A
出版信息
Proc Natl Acad Sci U S A. 1971 Dec;68(12):2962-6. doi: 10.1073/pnas.68.12.2962.
Abstract
Two new polarity suppressors (Su27 and Su78) have been isolated in Escherichia coli. Both suppress polarity in the trp and lac operons, and neither shows codon specificity for suppression. Extreme polar mutants of the lac z gene that contain either Su27 or Su78 make mRNA from the entire operon; however, no active beta-galactosidase is translated from the z-gene messenger, and the amount of distal messenger is greater than would be expected from corresponding enzyme activities. Puromycin treatment of lac(+) strains mimics the effect of polar chain-termination mutations in destabilizing lac mRNA. This effect is completely reversed by Su27, and it is partially reversed by Su78. The results suggest that these suppressors act by stabilizing mRNA after premature termination of protein synthesis.
摘要
在大肠杆菌中分离出了两种新的极性抑制因子（Su27和Su78）。它们都能抑制色氨酸和乳糖操纵子中的极性，且都不表现出抑制的密码子特异性。含有Su27或Su78的乳糖z基因的极端极性突变体可从整个操纵子产生信使核糖核酸（mRNA）；然而，z基因信使并未翻译出有活性的β-半乳糖苷酶，并且远端信使的量比相应酶活性预期的要多。用嘌呤霉素处理乳糖（+）菌株，在使乳糖mRNA不稳定方面模拟了极性链终止突变的作用。这种作用被Su27完全逆转，被Su78部分逆转。结果表明，这些抑制因子通过在蛋白质合成过早终止后稳定mRNA而起作用。
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