Ruckdeschel J C, Lininger L, Brzyski H, Miani M, Becker J
Transplantation. 1979 Sep;28(3):203-6. doi: 10.1097/00007890-197909000-00010.
Eight normal donors were serially tested over a period of 1 to 7 months for their in vitro lymphocyte responsiveness (IVLR) to 50 microgram of concanavalin A (Con A) per ml. 3H-Thymidine incorporation was monitored by routine scintillation counting and compared to actual numbers of proliferating cells (lymphoblasts) as measured by laser (flow) cytometry. Determination of total cell numbers or lymphoblast numbers was found to be superior (P less than 0.0001 and P less than 0.001, respectively) to 3H-thymidine incorporation as a measure of serial stability.
对8名正常供体在1至7个月的时间内进行了连续检测，以观察其体外淋巴细胞对每毫升50微克刀豆球蛋白A（伴刀豆球蛋白A）的反应性（IVLR）。通过常规闪烁计数监测3H-胸腺嘧啶核苷掺入情况，并与通过激光（流式）细胞术测量的增殖细胞（成淋巴细胞）实际数量进行比较。结果发现，作为连续稳定性的衡量指标，总细胞数或成淋巴细胞数的测定分别比3H-胸腺嘧啶核苷掺入更具优势（P分别小于0.0001和P小于0.001）。
