阿糖胞苷蛋白的纯化
Purification of the araC protein.
作者信息
Wilcox G, Clemetson K J, Santi D V, Englesberg E
出版信息
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2145-8. doi: 10.1073/pnas.68.9.2145.
Abstract
The araC gene product, a regulatory protein required for expression of the L-arabinose operon, has been purified by affinity chromatography on Sepharose 4B to which 4-aminophenyl-beta-D-6-deoxygalactopyranoside (an anti-inducer of the L-arabinose operon) had been covalently attached by means of a 4-aminophenylbutanamido side chain. Evidence is presented showing that the protein binds specifically to ara DNA.
摘要
阿拉伯糖操纵子表达所需的调节蛋白——阿拉伯糖操纵子阻遏蛋白（araC基因产物），已通过在共价连接了4-氨基苯基-β-D-6-脱氧吡喃半乳糖苷（一种阿拉伯糖操纵子的抗诱导剂）的琼脂糖4B上进行亲和层析而得到纯化，4-氨基苯基-β-D-6-脱氧吡喃半乳糖苷通过4-氨基苯基丁酰胺侧链共价连接。文中给出的证据表明该蛋白能特异性结合阿拉伯糖操纵子DNA。
相似文献
1
Purification of the araC protein.阿糖胞苷蛋白的纯化
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2145-8. doi: 10.1073/pnas.68.9.2145.
2
Isolation of araC protein by affinity chromatography.
Methods Enzymol. 1974;34:368-73. doi: 10.1016/s0076-6879(74)34040-2.
3
Regulation of the Escherichia coli L-arabinose operon studied by gel electrophoresis DNA binding assay.通过凝胶电泳DNA结合试验研究大肠杆菌L-阿拉伯糖操纵子的调控
J Mol Biol. 1984 Sep 25;178(3):611-28. doi: 10.1016/0022-2836(84)90241-9.
4
The Escherichia coli L-arabinose operon: binding sites of the regulatory proteins and a mechanism of positive and negative regulation.大肠杆菌L-阿拉伯糖操纵子：调节蛋白的结合位点及正负调控机制
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3346-50. doi: 10.1073/pnas.77.6.3346.
5
Purification and DNA-binding properties of the catabolite gene activator protein.分解代谢基因激活蛋白的纯化及DNA结合特性
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1222-5. doi: 10.1073/pnas.68.6.1222.
6
Mechanism of araC autoregulation and the domains of two overlapping promoters, Pc and PBAD, in the L-arabinose regulatory region of Escherichia coli.大肠杆菌L-阿拉伯糖调控区域中araC自动调节机制以及两个重叠启动子Pc和PBAD的结构域
Proc Natl Acad Sci U S A. 1981 Feb;78(2):752-6. doi: 10.1073/pnas.78.2.752.
7
Mutations in the araC regulatory gene of Escherichia coli B/r that affect repressor and activator functions of AraC protein.大肠杆菌B/r的araC调控基因中的突变，这些突变影响AraC蛋白的阻遏物和激活物功能。
J Bacteriol. 1986 Jun;166(3):892-900. doi: 10.1128/jb.166.3.892-900.1986.
8
Interaction of the regulatory gene product with the operator site in the L-arabinose operon of Escherichia coli.大肠杆菌L-阿拉伯糖操纵子中调控基因产物与操纵位点的相互作用。
J Mol Biol. 1974 Jan 5;85(4):589-602. doi: 10.1016/0022-2836(74)90317-9.
9
The DNA loop model for ara repression: AraC protein occupies the proposed loop sites in vivo and repression-negative mutations lie in these same sites.阿拉伯糖阻遏的DNA环模型：阿拉伯糖C蛋白在体内占据了所提出的环位点，且阻遏阴性突变位于这些相同位点。
Proc Natl Acad Sci U S A. 1986 Jun;83(11):3654-8. doi: 10.1073/pnas.83.11.3654.
10
Activation of ara operons by a truncated AraC protein does not require inducer.截短的AraC蛋白对ara操纵子的激活不需要诱导剂。
Proc Natl Acad Sci U S A. 1990 May;87(10):3708-12. doi: 10.1073/pnas.87.10.3708.
引用本文的文献
1
The araC regulatory gene mRNA contains a leader sequence.araC调控基因信使核糖核酸含有一个前导序列。
Mol Gen Genet. 1980;180(1):219-26. doi: 10.1007/BF00267373.
2
Genetic regulatory mechanisms and the ecological niche of Escherichia coli.大肠杆菌的遗传调控机制与生态位
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2453-5. doi: 10.1073/pnas.71.6.2453.
3
Characterization of strong polar mutations in a region immediately adjacent to the L-arabinose operator in Escherichia coli B-r.大肠杆菌B-r中紧邻L-阿拉伯糖操纵子区域的强极性突变的特征分析
J Bacteriol. 1972 Aug;111(2):383-91. doi: 10.1128/jb.111.2.383-391.1972.
4
Transcriptional control in the L-arabinose operon of Escherichia coli B-r.大肠杆菌B-r的L-阿拉伯糖操纵子中的转录调控
J Bacteriol. 1974 Apr;118(1):121-8. doi: 10.1128/jb.118.1.121-128.1974.
5
Structure and synthesis of the ribosomal ribonucleic acid of prokaryotes.原核生物核糖体核糖核酸的结构与合成
Bacteriol Rev. 1973 Dec;37(4):562-603. doi: 10.1128/br.37.4.562-603.1973.
6
Mutations in the araC regulatory gene of Escherichia coli B/r that affect repressor and activator functions of AraC protein.大肠杆菌B/r的araC调控基因中的突变，这些突变影响AraC蛋白的阻遏物和激活物功能。
J Bacteriol. 1986 Jun;166(3):892-900. doi: 10.1128/jb.166.3.892-900.1986.
7
lac repressor: 3-fluorotyrosine substitution for nuclear magnetic resonance studies.乳糖阻遏蛋白：用于核磁共振研究的3-氟酪氨酸取代
Proc Natl Acad Sci U S A. 1976 Oct;73(10):3471-5. doi: 10.1073/pnas.73.10.3471.
8
Design of molecular control mechanisms and the demand for gene expression.分子控制机制的设计与基因表达的需求。
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5647-51. doi: 10.1073/pnas.74.12.5647.
9
The site for catabolite deactivation in the L-arabinose BAD operon in Escherichia coli B/r.大肠杆菌B/r中L-阿拉伯糖BAD操纵子中分解代谢物失活的位点。
Arch Microbiol. 1976 Oct 11;110(1):135-43. doi: 10.1007/BF00416978.
本文引用的文献
1
NON-INDUCIBLE MUTANTS OF THE REGULATOR GENE IN THE "LACTOSE" SYSTEM OF ESCHERICHIA COLI.大肠杆菌“乳糖”系统中调节基因的非诱导型突变体
J Mol Biol. 1964 Apr;8:582-92. doi: 10.1016/s0022-2836(64)80013-9.
2
Chemical coupling of proteins to agarose.蛋白质与琼脂糖的化学偶联。
Nature. 1967 Sep 30;215(5109):1491-2. doi: 10.1038/2151491a0.
3
The purification of beta-galactosidase from Escherichia coli by affinity chromatography.通过亲和色谱法从大肠杆菌中纯化β-半乳糖苷酶。
J Biol Chem. 1971 Jan 10;246(1):196-200.
4
The L-rhamnose genetic system in Escherichia coli K-12.大肠杆菌K-12中的L-鼠李糖遗传系统。
Genetics. 1967 Mar;55(3):557-68. doi: 10.1093/genetics/55.3.557.
5
Further evidence for positive control of the L-arabinose system by gene araC.基因araC对L-阿拉伯糖系统进行正向调控的进一步证据。
J Mol Biol. 1967 May 14;25(3):443-54. doi: 10.1016/0022-2836(67)90197-0.
6
Positive control of enzyme synthesis by gene C in the L-arabinose system.在L-阿拉伯糖系统中，基因C对酶合成的正调控。
J Bacteriol. 1965 Oct;90(4):946-57. doi: 10.1128/jb.90.4.946-957.1965.
7
Selective enzyme purification by affinity chromatography.通过亲和色谱法进行选择性酶纯化。
Proc Natl Acad Sci U S A. 1968 Oct;61(2):636-43. doi: 10.1073/pnas.61.2.636.
8
D-Fucose as a gratuitous inducer of the L-arabinose operon in strains of Escherichia coli B-r mutant in gene araC.D-岩藻糖作为阿拉伯糖操纵子在大肠杆菌B-r araC基因突变株中的 gratuitous诱导剂 。 （注：这里“gratuitous”不太明确准确意思，可根据具体专业背景进一步确认准确含义来完善译文表述）
J Bacteriol. 1971 Jul;107(1):79-86. doi: 10.1128/jb.107.1.79-86.1971.
9
Nonsense mutants in the regulator gene araC of the L-arabinose system of Escherichia coli B-r.大肠杆菌B-r的L-阿拉伯糖系统调节基因araC中的无义突变体。
Genetics. 1970 May;65(1):27-39. doi: 10.1093/genetics/65.1.27.
10
Lac repressor-operator interaction. I. Equilibrium studies.乳糖阻遏蛋白与操纵基因的相互作用。I. 平衡研究。
J Mol Biol. 1970 Feb 28;48(1):67-83. doi: 10.1016/0022-2836(70)90219-6.
Zotero 插件