通过酵母中的抗生素抑制作用筛选核糖体突变体。
Selection of ribosomal mutants by antibiotic suppression in yeast.
作者信息
Bayliss F T, Vinopal R T
出版信息
Science. 1971 Dec 24;174(4016):1339-41. doi: 10.1126/science.174.4016.1339.
PMID:4944401
Abstract
Wild-type Saccharomyces cerevisiae is highly resistant to streptomycin. A histidine auxotroph was found which could grow without histidine in the presence of high concentrations of streptomycin. Selection for derivatives of this strain which could be suppressed by much lower concentrations of streptomycin yielded streptomycin-sensitive mutants which are cold-sensitive and have altered ribosomal profiles.
摘要
野生型酿酒酵母对链霉素具有高度抗性。发现了一种组氨酸营养缺陷型菌株，它在高浓度链霉素存在的情况下无需组氨酸就能生长。对该菌株的衍生物进行筛选，这些衍生物能够被低得多浓度的链霉素所抑制，从而得到了对链霉素敏感的突变体，这些突变体对温度敏感且核糖体图谱发生了改变。
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