在连续培养中培养鼠麻风分枝杆菌道格拉斯菌株。
Cultivation of the Douglas strain of Mycobacterium lepraemurium in continuous culture.
作者信息
Sula L, Dubina J
出版信息
Bull World Health Organ. 1971;45(2):209-12.
Abstract
Cultivation of Mycobacterium lepraemurium in cell-free media has not yet been successful, perhaps because of deterioration of the nutritional quality of the medium with prolonged incubation at 37 degrees C. Trials with a continuous culture technique, involving regular change of a simple liquid medium prepared from human placenta, demonstrated active fission of the Myco. lepraemurium cells. Abundant growth in the form by macroscopically visible colonies has not been achieved even after prolonged incubation up to 8 months.
摘要
尚未成功在无细胞培养基中培养鼠麻风杆菌，这可能是因为培养基在37℃下长时间孵育后营养质量下降。采用连续培养技术进行试验，即定期更换由人胎盘制备的简单液体培养基，结果显示鼠麻风杆菌细胞有活跃的分裂现象。即便长时间孵育长达8个月，也未实现以肉眼可见菌落形式的大量生长。
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本文引用的文献
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Elongation of a leprosy bacillus (Mycobacterium lepraemurium) in a cell-free medium.在无细胞培养基中麻风杆菌（鼠麻风分枝杆菌）的伸长。
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WHO CO-OPERATIVE STUDIES ON A SIMPLE CULTURE TECHNIQUE FOR THE ISOLATION OF MYCOBACTERIA. 1. PREPARATION, LYOPHILIZATION AND RECONSTITUTION OF A SIMPLE SEMI-SYNTHETIC CONCENTRATED LIQUID MEDIUM; CULTURE TECHNIQUE; GROWTH PATTERN OF DIFFERENT MYCOBACTERIA.世界卫生组织关于分枝杆菌分离的简单培养技术的合作研究。1. 一种简单的半合成浓缩液体培养基的制备、冻干和复溶；培养技术；不同分枝杆菌的生长模式。
Bull World Health Organ. 1963;29(5):589-606.
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Limited multiplication of M. lepraemurium in tissue culture.鼠麻风杆菌在组织培养中的增殖受限。
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The application of metabolic studies to leprosy research.代谢研究在麻风病研究中的应用。
Int J Lepr. 1954 Apr-Jun;22(2):147-61.
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