Mullan N A, Newsome P M, Cunnington P G, Palmer G H, Wilson M E
Infect Immun. 1974 Dec;10(6):1195-201. doi: 10.1128/iai.10.6.1195-1201.1974.
The ability of antisera to lipid A, induced in rabbits by immunization with lipid A complexed to various carriers, to protect mice against gram-negative infection and to inhibit the fluid loss caused by an enteropathogenic strain of Escherichia coli in the piglet ligated gut was investigated. No significant protection was obtained in either case, although passive hemolysis and quantitative precipitation tests showed the presence of antilipid A antibodies in the sera. Fluorescent antibody studies suggest that the lipid A is in a cryptic position on the surface of smooth strains of gram-negative bacteria.
研究了用与各种载体复合的脂多糖免疫家兔诱导产生的抗脂多糖血清保护小鼠抵抗革兰氏阴性菌感染以及抑制致病性大肠杆菌菌株在仔猪结扎肠段引起的液体流失的能力。尽管被动溶血和定量沉淀试验表明血清中存在抗脂多糖抗体,但在这两种情况下均未获得显著的保护作用。荧光抗体研究表明,脂多糖在革兰氏阴性菌光滑菌株表面处于隐蔽位置。