McCabe W R, Greely A
Infect Immun. 1973 Mar;7(3):386-92. doi: 10.1128/iai.7.3.386-392.1973.
Studies were carried out evaluating the protective activity of immunization with common enterobacterial antigen (CA) against challenge with heterologous gram-negative bacilli. Active immunization of mice with Escherichia coli 0:14 elicited titers of antibody to CA of 1:640 or greater but completely failed to enhance resistance to challenge by mouse virulent strains of Klebsiella pneumoniae or E. coli. Similarly, two lots, 324 and 422, of rabbit antisera to CA failed to afford passive protection to mice challenged with K. pneumoniae or E. coli. A third lot, 166, of rabbit antisera to CA did passively protect mice. The protective activity of antisera 166 was demonstrated to reflect its content of antibody to another cross-reactive antigen, Re determinant, of gram-negative bacilli which has previously been shown to protect against infections with heterologous bacilli rather than any protective effect of antibody to CA. These studies failed to demonstrate any protective activity of antibody to CA against challenge with heterologous CA containing gram-negative bacilli.
开展了多项研究，评估用普通肠杆菌抗原（CA）免疫对异源革兰氏阴性杆菌攻击的保护活性。用大肠杆菌0:14对小鼠进行主动免疫，可诱导出效价为1:640或更高的抗CA抗体，但完全未能增强对肺炎克雷伯菌或大肠杆菌小鼠强毒株攻击的抵抗力。同样，两批（324和422）兔抗CA血清未能为受到肺炎克雷伯菌或大肠杆菌攻击的小鼠提供被动保护。第三批（166）兔抗CA血清确实为小鼠提供了被动保护。已证明抗血清166的保护活性反映了其针对革兰氏阴性杆菌另一种交叉反应性抗原Re决定簇的抗体含量，此前已表明该抗原可预防异源杆菌感染，而非抗CA抗体的任何保护作用。这些研究未能证明抗CA抗体对含异源CA的革兰氏阴性杆菌攻击具有任何保护活性。
