淋巴囊肿病毒：在鲈形目鱼类细胞系中的分离与增殖
Lymphocystis virus: isolation and propagation in centrarchid fish cell lines.
作者信息
Wolf K, Gravell M, Malsberger R G
出版信息
Science. 1966 Feb 25;151(3713):1004-5. doi: 10.1126/science.151.3713.1004.
PMID:4955835
Abstract
A virus from fish with lymphocystis disease was isolated in fish cell cultures. Eleven serial transfers were made and the pathognomonic lymphocystis cells were produced in vitro in each transfer. Fish inoculated with 6th- and 9th-passage material developed the disease, and virus was reisolated front them.
摘要
从患有淋巴囊肿病的鱼身上分离出一种病毒，并在鱼类细胞培养物中进行培养。进行了11次连续传代，每次传代均在体外产生了具有病理特征性的淋巴囊肿细胞。用第6代和第9代传代材料接种的鱼患上了这种疾病，并且从它们身上重新分离出了病毒。
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