巨大芽孢杆菌中青霉素作用的初始结构损伤。
The initial structural lesion of penicillin action in Bacillus megaterium.
作者信息
Fitz-James P, Hancock R
出版信息
J Cell Biol. 1965 Aug;26(2):657-67. doi: 10.1083/jcb.26.2.657.
Abstract
The effect of penicillin on the structure of Bacillus megaterium cells was followed in media with and without osmotic stabilization. In peptone without osmotic support the cells showed a distortion of the normal membrane-wall relationship by 20 minutes. This appeared to be a combination of both membrane distortion and cytoplasmic leakage. Lytic changes quickly followed. With osmotic support a clean-cut lesion at the transverse-septal site developed by 10 minutes' growth in penicillin. The membrane lost its normal relationship to the cell wall and formed a pocket which was filled with a fibrous material which appeared to be unorganized wall mucopeptide. The pocket of fibers enlarged until the cell either lysed or formed a protoplast.
摘要
在有和没有渗透稳定作用的培养基中，研究了青霉素对巨大芽孢杆菌细胞结构的影响。在没有渗透支持的蛋白胨培养基中，20分钟内细胞就出现了正常膜 - 壁关系的扭曲。这似乎是膜扭曲和细胞质泄漏的共同作用。随后迅速出现溶解变化。在有渗透支持的情况下，在青霉素中生长10分钟后，横隔壁位点出现了明显的损伤。膜失去了与细胞壁的正常关系，形成了一个充满纤维状物质的袋状结构，该纤维状物质似乎是无组织的壁粘肽。纤维袋不断扩大，直到细胞裂解或形成原生质体。
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