Edwards J L, Busta F F, Speck M L
Appl Microbiol. 1965 Nov;13(6):858-64. doi: 10.1128/am.13.6.858-864.1965.
The following three criteria indicated that Bacillus subtilis A spores were injured, but not completely inactivated, by ultrahigh temperature treatment. (i) Significant reductions in survivors were observed when spores were enumerated with a standard medium but not when the medium contained added CaCl(2) and sodium dipicolinate. (ii) After a damaging heat treatment, more survivors were enumerated with the standard medium after incubation at 32 C than at 45 C, which was opposite to the result with untreated or slightly heated spores. (iii) Apparent numbers of survivors increased during the initial period of 3 C storage when enumerated with the standard medium at 45 C. No injury was evident when survivors were enumerated at either incubation temperature with the medium containing added CaCl(2) and sodium dipicolinate. Heat activation of the spores did not significantly influence the appearance of heat injury. The data suggested that the heat injury occurred in a germination system which was required in the absence of CaCl(2) and sodium dipicolinate.
以下三个标准表明，枯草芽孢杆菌A的孢子经超高温处理后受到损伤，但并未完全失活。(i) 当用标准培养基计数孢子时，存活菌数显著减少，但当培养基中添加了氯化钙和吡啶二羧酸二钠时则不然。(ii) 经过破坏性热处理后，在32℃培养后用标准培养基计数的存活菌数比在45℃培养后更多，这与未处理或轻微加热的孢子的结果相反。(iii) 当在45℃用标准培养基计数时，在3℃储存的初始阶段，存活菌的表观数量增加。当在任一培养温度下用添加了氯化钙和吡啶二羧酸二钠的培养基计数存活菌时，均未发现明显损伤。孢子的热激活对热损伤的表现没有显著影响。数据表明，热损伤发生在缺乏氯化钙和吡啶二羧酸二钠时所需的萌发系统中。
