Scheusner D L, Busta F F, Speck M L
Appl Microbiol. 1971 Jan;21(1):41-5. doi: 10.1128/am.21.1.41-45.1971.
Injury of test cultures was quantitated by differences in colony counts obtained with a complete medium and those obtained on conventional selective media. Staphylococcus aureus, Streptococcus faecalis, and several strains of Escherichia coli were injured when exposed to the quaternary ammonium compound methylalkyltrimethyl ammonium chloride. Representative hypochlorite sanitizers also caused injury of E. coli ML30. Sanitizer concentration appeared to be the main factor in the cause of death and injury, a higher concentration being needed to cause death. Increases in temperature did not result in substantial increases in injury; however, the lethal effect was greater at higher temperatures. Varying the cell concentration from 10(7) to 10(9) cells per ml did not change the fraction of cell population killed or injured. The inability or failure of common selective media to detect injured bacteria in food could have serious public health consequences.
通过在完全培养基上获得的菌落计数与在传统选择性培养基上获得的菌落计数之间的差异，对受试培养物的损伤进行定量。金黄色葡萄球菌、粪肠球菌和几种大肠杆菌菌株在接触季铵化合物甲基烷基三甲基氯化铵时会受到损伤。代表性的次氯酸盐消毒剂也会导致大肠杆菌ML30受到损伤。消毒剂浓度似乎是导致死亡和损伤的主要因素，导致死亡需要更高的浓度。温度升高并未导致损伤大幅增加；然而，在较高温度下致死效应更大。将细胞浓度从每毫升10⁷个细胞变化到10⁹个细胞，并未改变被杀灭或损伤的细胞群体比例。普通选择性培养基无法检测食品中受损细菌的情况可能会产生严重的公共卫生后果。
