Busta F F, Baillie E, Murrell W G
Appl Environ Microbiol. 1976 Aug;32(2):312-4. doi: 10.1128/aem.32.2.312-314.1976.
The addition of 0.6 M sucrose of 0.016 M Mg2+ to the enumeration medium was required for early expression of heat resistance (10 min at 70 degrees C) in stage V Bacillus cereus forspores. The addition of Mg2+ to the sporulation medium did not remove this requirment for sucrose of Mg2+. The heat damage did not affect forespore germination or outgrowth, but injured cells in the absence of sucrose or Mg2+ were not capable of cell division. The heat-induced sublethal damage apparently affected the forspore component(s) that could be repaired or was capable of normal function in the presence of added Mg2+ or sucrose.
在计数培养基中添加0.6 M蔗糖或0.016 M Mg2+，是蜡样芽孢杆菌V期芽孢早期表达耐热性（70℃处理10分钟）所必需的。向芽孢形成培养基中添加Mg2+并不能消除对蔗糖或Mg2+的这一需求。热损伤并不影响前芽孢的萌发或生长，但在没有蔗糖或Mg2+的情况下，受损细胞无法进行细胞分裂。热诱导的亚致死损伤显然影响了前芽孢成分，在添加Mg2+或蔗糖的情况下，这些成分可以修复或具备正常功能。
