Holm G
Clin Exp Immunol. 1966 Jan;1(1):45-60.
A nephrotic syndrome was induced in randomly bred Sprague-Dawley rats by repeated intraperitoneal injections of homologous kidney extract and Freund's complete adjuvant. Cell injury was measured by estimating the release of radioactivity from damaged target cells labelled with C-thymidine. Various conditions influencing the reproducibility of the isotope method were investigated. Blood lymphoid cells, lymph node cells and spleen cells from these rats damaged primary monolayer cultures of rat kidney cells after 18 hr incubation. Lymphoid cells from liver sensitized rats were also cytotoxic to rat kidney cells, whereas lymphoid cells from rats injected with other rat tissue extracts (lung or spleen) or given only adjuvant did not react. Primary cultures of rat lung cells were sensitive while cells of a rat liver cell strain were resistant to the cytotoxic action of lymphoid cells from kidney sensitized rats. Sera from nephrotic rats did not damage rat kidney cells under the experimental conditions used here.
通过反复腹腔注射同源肾提取物和弗氏完全佐剂,在随机繁殖的斯普拉格 - 道利大鼠中诱发肾病综合征。通过估计用碳 - 胸腺嘧啶标记受损靶细胞的放射性释放来测量细胞损伤。研究了影响同位素方法重现性的各种条件。这些大鼠的血液淋巴细胞、淋巴结细胞和脾细胞在孵育18小时后损伤了大鼠肾细胞的原代单层培养物。来自肝脏致敏大鼠的淋巴细胞对大鼠肾细胞也具有细胞毒性,而注射其他大鼠组织提取物(肺或脾)或仅给予佐剂的大鼠的淋巴细胞则无反应。大鼠肺细胞的原代培养物敏感,而大鼠肝细胞系的细胞对来自肾致敏大鼠的淋巴细胞的细胞毒性作用具有抗性。在此处使用的实验条件下,肾病大鼠的血清不会损伤大鼠肾细胞。