用于估算微生物细胞体积的同位素方法。
Isotopic method for estimating microbial cell volumes.
作者信息
Reid D F, Frank H A
出版信息
J Bacteriol. 1966 Sep;92(3):639-44. doi: 10.1128/jb.92.3.639-644.1966.
Abstract
Reid, Della F. (University of Hawaii, Honolulu), and Hilmer A. Frank. Isotopic method for estimating microbial cell volumes. J. Bacteriol. 92:639-644. 1966.-A sensitive isotopic method was developed for estimating the mean cell volume in small numbers of bacterial spores by measuring directly the extracellular fluid volume when cells are present in a saturated inulin solution containing inulin-C(14) at low specific activity. The method minimizes errors due to solute adsorption to the cells and avoids errors arising from differences in cell packing. The effects of these sources of error on cell volume and intercellular space estimations made with nonpenetrating solutes are discussed.
摘要
里德，德拉·F.（夏威夷大学，檀香山），以及希尔默·A.弗兰克。估算微生物细胞体积的同位素方法。《细菌学杂志》92:639 - 644。1966年。——开发了一种灵敏的同位素方法，通过在含有低比活度菊粉 - C(14)的饱和菊粉溶液中，当细胞存在时直接测量细胞外液体积，来估算少量细菌孢子的平均细胞体积。该方法将溶质吸附到细胞上导致的误差降至最低，并避免了因细胞堆积差异而产生的误差。讨论了这些误差来源对用非渗透性溶质进行细胞体积和细胞间隙估算的影响。
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