Watson R W, Levinson M L
Appl Microbiol. 1967 Mar;15(2):398-402. doi: 10.1128/am.15.2.398-402.1967.
A rapid procedure for the quantitative determination of extracellular water in bulk bakers' yeast was developed on the basis of the solute dilution principle. A reagent is prepared by synthesizing the diazonium ion of p-aminobenzoic acid and coupling it to peptone. This "azopeptone reagent" permits direct colorimetric measurement, which accounts for the rapidity and simplicity of the test. Potential errors due to osmotic effects are avoided by supplementing the reagent with saline and, more importantly, minimizing the duration of contact between reagent and cells. The new method has acceptable accuracy and precision, and may also be suitable for use with other microorganisms.
基于溶质稀释原理，开发了一种快速定量测定面包酵母细胞外水的方法。通过合成对氨基苯甲酸的重氮离子并将其与蛋白胨偶联来制备试剂。这种“偶氮蛋白胨试剂”允许直接比色测量，这说明了该测试的快速性和简易性。通过在试剂中添加盐水，更重要的是尽量缩短试剂与细胞的接触时间，避免了因渗透效应引起的潜在误差。新方法具有可接受的准确度和精密度，也可能适用于其他微生物。
