过氧化物酶和1,5 - 二氨基戊烷对豌豆幼苗二胺氧化酶的失活作用
The inactivation of pea-seedling diamine oxidase by peroxidase and 1,5-diaminopentane.
作者信息
Hill J M
出版信息
Biochem J. 1967 Sep;104(3):1048-55. doi: 10.1042/bj1041048.
Abstract
- The rate of oxidative deamination of 1,5-diaminopentane by pea-seedling extracts, which contain diamine oxidase [diamine-oxygen oxidoreductase (deaminating), EC 1.4.3.6], was increased by adding pyridoxal or pyridoxal phosphate. 2. Evidence was obtained that pyridoxal does not activate the apoenzyme of diamine oxidase, but prevents the inactivation of the enzyme. 3. This inactivation only occurred when 1,5-diaminopentane was the substrate and depended on a second thermolabile factor in the extract besides the diamine oxidase. 4. Purified diamine oxidase, when catalysing the oxidation of 1,5-diaminopentane, was rapidly inactivated in the presence of peroxidase. 5. The inactivation was prevented not only by pyridoxal and pyridoxal phosphate but also by several unrelated compounds including alpha-oxoglutarate, catechol and o-aminobenzaldehyde. 6. It is suggested that peroxidase catalyses the further oxidation of the product of the oxidative deamination of 1,5-diaminopentane to a compound that inactivates diamine oxidase. 7. The results diminish the relevance of previous evidence that plant diamine oxidase contains pyridoxal phosphate.
摘要
- 含有二胺氧化酶[二胺-氧氧化还原酶（脱氨基），EC 1.4.3.6]的豌豆幼苗提取物对1,5-二氨基戊烷的氧化脱氨速率，通过添加吡哆醛或磷酸吡哆醛而增加。2. 有证据表明，吡哆醛不会激活二胺氧化酶的脱辅基酶，但能防止该酶失活。3. 这种失活仅在以1,5-二氨基戊烷为底物时发生，并且除了二胺氧化酶外，还取决于提取物中的第二个不耐热因子。4. 纯化的二胺氧化酶在催化1,5-二氨基戊烷氧化时，在过氧化物酶存在下会迅速失活。5. 不仅吡哆醛和磷酸吡哆醛，而且包括α-酮戊二酸、儿茶酚和邻氨基苯甲醛在内的几种不相关化合物都能防止这种失活。6. 有人提出，过氧化物酶催化1,5-二氨基戊烷氧化脱氨产物进一步氧化为一种使二胺氧化酶失活的化合物。7. 这些结果降低了先前关于植物二胺氧化酶含有磷酸吡哆醛的证据的相关性。
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本文引用的文献
1
The oxidation of amines by extracts of pea seedlings.豌豆幼苗提取物对胺类的氧化作用。
Biochem J. 1952 Jan;50(3):360-9. doi: 10.1042/bj0500360.
2
Mode of action of isonicotinic hydrazide.异烟肼的作用方式。
Nature. 1955 Jul 2;176(4470):34-5. doi: 10.1038/176034a0.
3
PYRIDOXAL PHOSPHATE AS A CONSTITUENT OF THE HISTAMINASE (BENZYLAMINE OXIDASE) OF PIG PLASMA.磷酸吡哆醛作为猪血浆组胺酶（苄胺氧化酶）的一个组成成分
Proc R Soc Lond B Biol Sci. 1965 Aug 24;163:45-60. doi: 10.1098/rspb.1965.0059.
4
BENZYLAMINE OXIDASE AND HISTAMINASE: PURIFICATION AND CRYSTALLIZATION OF AN ENZYME FROM PIG PLASMA.苄胺氧化酶和组胺酶：猪血浆中一种酶的纯化与结晶
Proc R Soc Lond B Biol Sci. 1964 Dec 15;161:153-67. doi: 10.1098/rspb.1964.0086.
5
MONOAMINE OXIDASE. IV. NATURE OF THE SECOND PROSTHETIC GROUP OF PLASMA MONOAMINE OXIDASE.单胺氧化酶。IV. 血浆单胺氧化酶第二种辅基的性质。
J Biol Chem. 1963 Aug;238:2669-75.
6
Monoamine oxidase. I. Purification, crystallization, and properties of plasma monoamine oxidase.单胺氧化酶。I. 血浆单胺氧化酶的纯化、结晶及性质
J Biol Chem. 1962 May;237:1511-6.
7
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8
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9
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10
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