van Ramshorst J D
Bull World Health Organ. 1967;36(2):209-18.
A study was made of the concentration procedures which are necessary in order to determine the D-antigen content of poliomyelitis vaccines by means of the gel-diffusion method. The most suitable methods were considered to be the aluminium-phosphate method developed by Fantes, and ultrafiltration. The AlPO(4) method was found to be superior in precision to ultrafiltration, but the latter was more convenient for small samples. The AlPO(4) method usually gave about 10%-20% lower D-antigen values than the filtration method, probably owing to incomplete elution of antigen from the precipitate.A two-step concentration procedure involving AlPO(4) precipitation followed by ultrafiltration was employed for determining the D-antigen content of combined and/or adsorbed vaccines.The importance of the different variables which occur in D-antigen determination by these methods is discussed.
为了用凝胶扩散法测定脊髓灰质炎疫苗的D抗原含量，对所需的浓缩方法进行了研究。最适合的方法被认为是Fantes开发的磷酸铝法和超滤法。发现磷酸铝法在精度上优于超滤法，但超滤法对小样本更方便。磷酸铝法通常比过滤法得到的D抗原值低约10%-20%，这可能是由于抗原从沉淀物中洗脱不完全。采用两步浓缩程序，先进行磷酸铝沉淀，然后超滤，以测定联合疫苗和/或吸附疫苗的D抗原含量。讨论了这些方法在D抗原测定中出现的不同变量的重要性。
