人氧合血红蛋白在聚乙二醇溶液中的结晶
Crystallization of human oxyhaemoglobin from poly(ethylene glycol) solutions.
作者信息
Grabowski M J, Brzozowski A M, Derewenda Z S, Skarźnński T, Cygler M, Stepień A, Derewenda A E
出版信息
Biochem J. 1978 Apr 1;171(1):277-9. doi: 10.1042/bj1710277.
Crystals of human oxyhaemoglobin were obtained from poly(ethylene glycol) solutions. Spectroscopic and spectrophotometric measurements on the solutions during crystallization and on the dissolved crystals indicate that the method yields stable crystals of oxyhaemoglobin. Preliminary X-ray studies showed that the crystals obtained are isomorphous with those of deoxyhaemoglobin obtained from poly(ethylene glycol) solutions [Ward, Wishner, Lattman & Love (1975) J. Mol. Biol. 98, 161-177].
人氧合血红蛋白晶体是从聚乙二醇溶液中获得的。在结晶过程中对溶液以及对溶解后的晶体进行光谱和分光光度测量表明，该方法可得到稳定的氧合血红蛋白晶体。初步的X射线研究表明，所获得的晶体与从聚乙二醇溶液中获得的脱氧血红蛋白晶体同晶型[沃德、威斯纳、拉特曼和洛夫（1975年）《分子生物学杂志》98卷，第161 - 177页]。
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