Aoki T, Takahashi T
J Exp Med. 1972 Mar 1;135(3):443-57. doi: 10.1084/jem.135.3.443.
IMMUNOELECTRON MICROSCOPY (IEM) OF MOUSE CELLS WHICH WERE PRODUCTIVELY INFECTED WITH MURINE LEUKEMIA VIRUS (MULV) YIELDED THE FOLLOWING CONCLUSIONS: See PDF for Structure With two exceptions, the alloantigens H-2, theta, Ly-A, and Ly-B were not present on complete or incomplete virions produced by cells bearing these antigens.The exceptions were H-2(k) (but not H-2(b)) and theta both appeared on only a minority of virions and never on more than a small part of the circumference. The incidental discovery of an additional envelope antigen on virions produced by a BALB/c mouse myeloma but lacking from passage A Gross virions distinguishes these two viruses as MuLV subtypes; it also illustrates that IEM can be applied as a primary tool for antigenic analysis, as well as for its usual purpose of finding out where antigens are situated.
对被小鼠白血病病毒(MuLV)有效感染的小鼠细胞进行免疫电子显微镜(IEM)检测得出以下结论:结构见PDF 除两个例外情况外,携带这些抗原的细胞产生的完整或不完整病毒粒子上不存在同种异体抗原H-2、θ、Ly-A和Ly-B。例外情况是H-2(k)(而非H-2(b))和θ仅出现在少数病毒粒子上,且从未出现在超过一小部分的病毒粒子圆周上。在一只BALB/c小鼠骨髓瘤产生的病毒粒子上偶然发现了一种额外的包膜抗原,而传代A Gross病毒粒子上没有这种抗原,这将这两种病毒区分为MuLV亚型;这也表明IEM可作为抗原分析的主要工具,以及用于其通常的确定抗原位置的目的。