蚊子DNA的甲基化

Methylation of mosquito DNA.

作者信息

Adams R L, McKay E L, Craig L M, Burdon R H

出版信息

Biochim Biophys Acta. 1979 Jun 20;563(1):72-81. doi: 10.1016/0005-2787(79)90008-x.

Abstract

Cells of the mosquito Aedes albopictus have 5.6 pg DNA/cell. This DNA is 58 mol% (A + T) and has about 0.03 mol% each of 5-methylcytosine and 6-methylaminopurine. The DNA is largely degraded by the restriction enzyme HpaII showing the virtual absence of the sequence CMeCGG. Small amounts of enzymic activity recovered largely in non-nuclear fractions transfer methyl groups from S-adenosylmethionine to cytosine and adenine.

摘要

白纹伊蚊细胞的DNA含量为5.6 pg/细胞。该DNA中（A+T）的摩尔含量为58%，5-甲基胞嘧啶和6-甲基腺嘌呤的摩尔含量均约为0.03%。该DNA在很大程度上被限制性内切酶HpaII降解，表明几乎不存在CMeCGG序列。少量的酶活性主要在非核组分中检测到，可将S-腺苷甲硫氨酸的甲基转移至胞嘧啶和腺嘌呤上。