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DNA甲基化酶对表观限制性内切酶识别特异性的改变。

Alteration of apparent restriction endonuclease recognition specificities by DNA methylases.

作者信息

Nelson M, Christ C, Schildkraut I

出版信息

Nucleic Acids Res. 1984 Jul 11;12(13):5165-73. doi: 10.1093/nar/12.13.5165.

DOI:10.1093/nar/12.13.5165
PMID:6087274
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC318911/
Abstract

An in vitro method of altering the apparent cleavage specificities of restriction endonucleases was developed using DNA modification methylases. This method was used to reduce the number of cleavage sites for 34 restriction endonucleases. In particular, single-site cleavages were achieved for Nhe I in Adeno-2 DNA and for Acc I and Hinc II in pBR322 DNA by specifically methylating all but one recognition sequence.

摘要

利用DNA修饰甲基化酶开发了一种体外改变限制性内切核酸酶表观切割特异性的方法。该方法用于减少34种限制性内切核酸酶的切割位点数量。具体而言，通过特异性甲基化除一个识别序列外的所有序列，在腺病毒2型DNA中实现了Nhe I的单一位点切割，在pBR322 DNA中实现了Acc I和Hinc II的单一位点切割。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ab9/318911/1ba88c95d8b2/nar00331-0036-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ab9/318911/c6e5f5f884fc/nar00331-0032-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ab9/318911/510588706a41/nar00331-0033-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ab9/318911/1ba88c95d8b2/nar00331-0036-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ab9/318911/c6e5f5f884fc/nar00331-0032-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ab9/318911/510588706a41/nar00331-0033-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ab9/318911/1ba88c95d8b2/nar00331-0036-a.jpg

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本文引用的文献

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The effect of site specific methylation on restriction endonuclease cleavage (update).位点特异性甲基化对限制性内切酶切割的影响（更新）。
Nucleic Acids Res. 1983 Jan 11;11(1):r169-73. doi: 10.1093/nar/11.1.235-c.
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Restriction and modification in Bacillus subtilis: DNA methylation potential of the related bacteriophages Z, SPR, SP beta, phi 3T, and rho 11.
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Punctuated distribution of recombination hotspots and demarcation of pericentromeric regions in Phaseolus vulgaris L.菜豆中重组热点的间断分布及着丝粒周围区域的划分
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Enzymes used in molecular biology: a useful guide.分子生物学中使用的酶：实用指南。
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Cloning of CviPII nicking and modification system from chlorella virus NYs-1 and application of Nt.CviPII in random DNA amplification.从绿藻病毒 NYs-1 克隆 CviPII 切口和修饰系统及 Nt.CviPII 在随机 DNA 扩增中的应用
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