Elson C J, Carter S D, Scott D G, Bacon P A, Lowe J
Rheumatol Int. 1985;5(4):175-9. doi: 10.1007/BF00541519.
A new assay for IgGRF activity is described which employs human IgGFc as the antigen and a radiolabelled monoclonal antibody directed against human IgG (CHI domain) as the developing antibody. Using this assay IgGRF activity against human IgG isotypes was measured and most sera from RA patients were shown to react predominantly with IgG1 and IgG2 but few reacted with IgG3 and IgG4. The same sera were tested for IgMRF to the IgG isotypes. IgG2 was the best antigen, IgG1 and IgG4 were less so and reactivity with IgG3 was the lowest. IgGRF without associated IgMRF was obtained, its specificity compared to that of IgMRF, and found to be broadly similar. With the new assay high levels of serum IgGRF were found in those RA patients with extraarticular disease but not in RA patients with synovitis alone.
本文描述了一种新的IgGRF活性检测方法，该方法以人IgGFc作为抗原，以针对人IgG（CHI结构域）的放射性标记单克隆抗体作为显色抗体。利用该检测方法测定了针对人IgG各亚型的IgGRF活性，结果显示大多数类风湿关节炎（RA）患者的血清主要与IgG1和IgG2发生反应，但很少与IgG3和IgG4发生反应。对相同的血清检测了针对IgG各亚型的IgMRF。IgG2是最佳抗原，IgG1和IgG4次之，与IgG3的反应性最低。获得了不伴有IgMRF的IgGRF，将其特异性与IgMRF的特异性进行比较，发现二者大致相似。通过这种新的检测方法，在患有关节外疾病的RA患者中发现了高水平的血清IgGRF，而仅患有滑膜炎的RA患者中未发现。
