使用库尔特计数器进行红细胞总数和体积分布计数时的一些问题。
Some problems in the use of the Coulter counter for erythrocyte total counts and volume distribution.
作者信息
Collier H B
出版信息
J Clin Pathol. 1968 Mar;21(2):179-82. doi: 10.1136/jcp.21.2.179.
Normal erythrocytes were subject to swelling and some haemolysis when suspended in a NaCl-formaldehyde-Na(2)EDTA diluent of pH 4.9 for routine blood-cell counting in the Coulter counter. A phosphate-buffered saline of pH 7.4 was substituted and has proven satisfactory for total erythrocyte counts. Polyvinyltoluene latex was unsuitable for volume calibration of the counter, because of aggregation of the particles in saline. Ragweed pollen grains gave a calibration factor that agreed well with that for fresh erythrocytes. Recent batches of Dade's Erythro-Trol (a suspension of fixed erythrocytes) were satisfactory for both total counts and volume calibration.
当正常红细胞悬浮于pH 4.9的氯化钠-甲醛-Na₂EDTA稀释液中，用于库尔特计数器进行常规血细胞计数时，会发生肿胀并出现一些溶血现象。现用pH 7.4的磷酸盐缓冲盐水替代，结果表明该缓冲液用于红细胞总数计数效果令人满意。聚乙烯甲苯乳胶不适用于计数器的体积校准，因为颗粒在盐水中会发生聚集。豚草花粉粒给出的校准因子与新鲜红细胞的校准因子非常吻合。最近几批达德公司的红细胞校准物（固定红细胞悬液）用于总数计数和体积校准均令人满意。
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