Kwant W O, Seeman P
J Gen Physiol. 1970 Feb;55(2):208-19. doi: 10.1085/jgp.55.2.208.
The osmotic swelling of intact erythrocytes in hypotonic solutions was measured using microhematocrit tubes, Van Allen tubes, and a calibrated Coulter counter. In agreement with earlier workers the intact cells did not behave as perfect osmometers, the cells swelling less than predicted by the Boyle-van't Hoff law. Erythrocyte ghosts were prepared from fresh intact erythrocytes by one-step hemolysis in 0.25% NaCl at an extremely dilute concentration of cells and the membranes were sealed at 37 degrees . The ghosts were mixed with NaCl solutions of different osmolarities and the MCV (mean cell volume) of the shrunken cells immediately monitored by a calibrated Coulter counter. It was found that the MCV values of the shrunken ghosts were accurately predicted by the Boyle-van't Hoff law. These results indicate that these erythrocyte ghosts behaved as perfect osmometers.
使用微量血细胞比容管、范·艾伦管和校准的库尔特计数器测量完整红细胞在低渗溶液中的渗透性肿胀。与早期研究者的结果一致，完整细胞的行为并不像理想的渗透压计，细胞肿胀程度小于玻意耳-范托夫定律预测的程度。通过在极低细胞浓度的0.25%氯化钠中一步溶血，从新鲜完整红细胞制备红细胞影，并在37摄氏度密封膜。将红细胞影与不同渗透压的氯化钠溶液混合，并立即通过校准的库尔特计数器监测收缩细胞的平均细胞体积（MCV）。发现收缩红细胞影的MCV值可由玻意耳-范托夫定律准确预测。这些结果表明这些红细胞影表现为理想的渗透压计。
