Calloway D H, Kumar A M
Appl Microbiol. 1969 Jan;17(1):176-8. doi: 10.1128/am.17.1.176-178.1969.
Hydrogenomonas eutropha cells harvested from semicontinuous autotrophic culture and washed free of substrate contain about 13% of nitrogen on a dry-solids basis. Biological value and digestibility of the bacterial nitrogen were determined in the rat by use of an abbreviated Mitchell-Thomas nitrogen balance technique and casein as the standard protein. Casein nitrogen was 99% digestible, and that of both whole boiled and sonically ruptured bacterial cells was 93%. Biological value of casein and the bacterial preparations was uniformly 77%. Amino acid composition of the bacteria, as in the case of casein, indicates a first limitation of sulfur-containing amino acids. These compositional features suggest that H. eutropha may be potentially valuable as a protein supplement in animal feeds.
从半连续自养培养物中收获并洗净底物的真养产碱菌细胞，以干固体计含氮量约为13%。利用简化的米切尔 - 托马斯氮平衡技术，并以酪蛋白作为标准蛋白质，在大鼠身上测定了细菌氮的生物价值和消化率。酪蛋白氮的消化率为99%，全煮细菌细胞和经超声破碎的细菌细胞的消化率均为93%。酪蛋白和细菌制剂的生物价值均为77%。与酪蛋白一样，细菌的氨基酸组成表明含硫氨基酸是首要限制因素。这些组成特征表明，真养产碱菌作为动物饲料中的蛋白质补充剂可能具有潜在价值。
