Repaske R, Mayer R
Appl Environ Microbiol. 1976 Oct;32(4):592-7. doi: 10.1128/aem.32.4.592-597.1976.
Alcaligenes eutrophus was grown autotrophically in 23-liter batch cultures in a controlled H2-O2-CO2 atmosphere. It was demonstrated that the need for periodic supplements of individual nutrients could be anticipated before cell growth depleted these nutrients to the point of becoming growth rate limiting. As a result, exponential growth was extended to optical densities of 44, with doubling times maintained at 2 h. Cultures having an initial optical density of 0.040 to 0.70 reached the final optical density of 60 in about 25 h. The final viable count was 1.2 X 10(11) cells per ml, and the dry weight was 25 g/liter.
在可控的氢气-氧气-二氧化碳气氛中，在23升的分批培养物中自养培养真养产碱菌。结果表明，在细胞生长将这些营养物质消耗到成为生长速率限制因素之前，就可以预测到需要定期补充个别营养物质。因此，指数生长期延长至光密度为44，倍增时间维持在2小时。初始光密度为0.040至0.70的培养物在约25小时内达到最终光密度60。最终活细胞计数为每毫升1.2×10¹¹个细胞，干重为25克/升。
