Gray R L, Wenzel F J, Emanuel D A
Appl Microbiol. 1969 Mar;17(3):454-6. doi: 10.1128/am.17.3.454-456.1969.
Farmer's lung is a serious disabling pulmonary disease found in agricultural workers. The disease is believed to be a hypersensitivity to the thermophilic actinomycetes, principally Thermopolyspora polyspora. This organism is difficult to stain with the usual bacteriological stains and thus far has not been demonstrated in the lung tissue by microscopic methods. In this paper, it is demonstrated that the fluorescent-antibody technique is a simple method for the positive identification of T. polyspora. The technique can also be used as a rapid screening test for the detection of antibodies to T. polyspora in the patient's serum. In addition, it opens up the possibility of the identification of T. polyspora in the lung tissue of patients with farmer's lung and makes available a means for the study of the immunological reaction in the lung parenchyma. No false positive or cross-reactions with Thermoactinomyces vulgaris or Streptomyces griseus could be demonstrated.
农民肺是一种在农业工人中发现的严重致残性肺部疾病。该病被认为是对嗜热放线菌，主要是嗜热栖热放线菌的一种超敏反应。这种微生物很难用常规细菌学染色法染色，到目前为止，尚未通过显微镜方法在肺组织中证实其存在。本文证明，荧光抗体技术是一种阳性鉴定嗜热栖热放线菌的简单方法。该技术还可用作快速筛查试验，以检测患者血清中针对嗜热栖热放线菌的抗体。此外，它为在农民肺患者的肺组织中鉴定嗜热栖热放线菌提供了可能性，并为研究肺实质中的免疫反应提供了一种手段。未发现与普通嗜热放线菌或灰色链霉菌有假阳性或交叉反应。
