牛过敏性肺炎的免疫荧光研究。
Immunofluorescent studies of bovine hypersensitivity pneumonitis.
作者信息
Wilkie B N, Gygax M, Pauli B
出版信息
Can J Comp Med. 1974 Oct;38(4):475-9.
Abstract
Antigens of Micropolyspora faeni were located by immunofluorescence in apparent association with alveolar macrophages within sections of lung from cases of bovine interstitial pneumonia. Pretreatment of frozen sections with pH 2.8 glycine-HC1 buffer was a necessary prerequisite for specific staining. Bovine immunoglobulin and bovine complement were identified in similar locations.
摘要
通过免疫荧光法在患牛间质性肺炎病例的肺切片中发现，微小多孢菌抗原明显与肺泡巨噬细胞相关。用pH 2.8甘氨酸 - 盐酸缓冲液预处理冰冻切片是特异性染色的必要前提。在相似位置鉴定出牛免疫球蛋白和牛补体。
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