Naitoh Y
J Gen Physiol. 1969 May;53(5):517-29. doi: 10.1085/jgp.53.5.517.
The predominant orientation of cilia in glycerol-extracted Paramecium is toward the posterior of the specimen in a KCl solution. The cilia became reoriented toward the anterior shortly after transfer of the extracted cell to a mixture of ATP, calcium, and zinc. The degree of response was graded as a function of the concentration of each of the three essential factors. Minimum concentrations for the maximum response were 0.2 mM in ATP, 0.8 mM in calcium, and 0.0002 mM in zinc. The observations support the hypothesis that cation-induced ciliary reversal in live specimens is initiated by calcium ions which become displaced from an inferred cellular cation exchanger system.
在氯化钾溶液中,甘油提取的草履虫中纤毛的主要取向是朝向标本的后部。将提取的细胞转移到ATP、钙和锌的混合物中后不久,纤毛就重新朝向了前部。反应程度根据这三种必需因子各自的浓度进行分级。产生最大反应的最低浓度分别为:ATP中0.2 mM,钙中0.8 mM,锌中0.0002 mM。这些观察结果支持了这样一种假说,即活标本中阳离子诱导的纤毛逆转是由从推测的细胞阳离子交换系统中置换出来的钙离子引发的。