体内细胞脱氧核糖核酸的紫外线改变

Tan E M, Stoughton R B

Proc Natl Acad Sci U S A. 1969 Mar;62(3):708-14. doi: 10.1073/pnas.62.3.708.

DNA after irradiation with ultraviolet light was immunogenic in rabbits and elicited serum antibodies reacting specifically with UV-irradiated DNA. The serological reactions were demonstrated by immunodiffusion, complement fixation, and immunofluorescence. By immunofluorescence, antisera reacted with cell nuclei of irradiated tissue sections but not with unirradiated tissue. This method was employed to show the presence of UV lesions in tissues of mice exposed to UV light. UV lesions in DNA were present in nuclei of epidermal cells, and in heavily irradiated animals they were also detected in the corium immediately below the epidermis. The method is useful not only for directly demonstrating UV lesions of DNA but also for localizing such lesions in tissues.

紫外线照射后的DNA对兔子具有免疫原性，并能引发与紫外线照射的DNA特异性反应的血清抗体。这些血清学反应通过免疫扩散、补体结合和免疫荧光得以证实。通过免疫荧光法，抗血清与照射过的组织切片的细胞核发生反应，但不与未照射的组织发生反应。该方法被用于显示暴露于紫外线的小鼠组织中紫外线损伤的存在。DNA中的紫外线损伤存在于表皮细胞核中，在受重度照射的动物中，在紧挨着表皮下方的真皮中也检测到了这种损伤。该方法不仅可用于直接证明DNA的紫外线损伤，还可用于在组织中定位此类损伤。