Maverakis N H, Wiley B B
J Bacteriol. 1969 Aug;99(2):472-9. doi: 10.1128/jb.99.2.472-479.1969.
The Smith diffuse variant and the wound mucoid strain of Staphylococcus aureus were shown to exhibit serologically distinct capsules. The Welwood and K-6 strains of S. aureus were tested to determine their capsular types. Both Welwood and K-6 were found to be representative of the Smith capsular type. An additional 13 isolates of S. aureus from mice were tested. Gel double-diffusion tests and immunoelectrophoresis of staphylococcal antigens disclosed the possible existence of at least two additional capsular types. Passive hemagglutination tests carried out with cells sensitized with 1 mg of antigen per ml showed a multiplicity of cross-reacting antigens. However, cells sensitized either with 0.1 or 0.05 mg of antigen per ml and reacted with antisera absorbed with 10 or 1 mug/ml showed the presence of a specific antigen in each strain of S. aureus. Corroborative evidence for a multiplicity of capsular types was obtained by the specific capsular reaction. At least four capsular types of S. aureus were found. The prototypic strains for these antigens are the RLM or wound strain, the Smith diffuse strain, and mouse strains designated 36T and 43R. We propose to designate these types 1, 2, 3, and 4, respectively.
已证明金黄色葡萄球菌的史密斯弥漫型变种和伤口黏液型菌株表现出血清学上不同的荚膜。对金黄色葡萄球菌的韦尔伍德菌株和K - 6菌株进行了测试，以确定它们的荚膜类型。发现韦尔伍德菌株和K - 6菌株均代表史密斯荚膜型。对另外13株从小鼠分离出的金黄色葡萄球菌进行了测试。葡萄球菌抗原的凝胶双向扩散试验和免疫电泳揭示了可能至少还存在两种其他荚膜类型。用每毫升含1毫克抗原致敏的细胞进行的被动血凝试验显示存在多种交叉反应抗原。然而，用每毫升含0.1或0.05毫克抗原致敏且与用10或1微克/毫升吸收的抗血清反应的细胞显示，每种金黄色葡萄球菌菌株中都存在一种特异性抗原。通过特异性荚膜反应获得了关于多种荚膜类型的确证证据。发现至少有四种金黄色葡萄球菌荚膜类型。这些抗原的原型菌株是RLM或伤口菌株、史密斯弥漫型菌株以及命名为36T和43R的小鼠菌株。我们提议分别将这些类型命名为1型、2型、3型和4型。
