Yoshida K, Ichiman Y, Umeda A
Department of Microbiology, St. Marianna University School of Medicine, Kawasaki, Japan.
J Appl Bacteriol. 1988 Dec;65(6):491-9. doi: 10.1111/j.1365-2672.1988.tb01922.x.
Strain ST67P of Staphylococcus hyicus grew diffusely in regular serum-soft agar. With the addition of rabbit antisera prepared with Staph. aureus strains, Smith, NS58D or NS41D, capsular type A, B or C, respectively, the organisms converted to compact type growth. Mice immunized with heat-killed vaccine of strain ST67P showed significant resistance against either homologous or heterologous strains, Smith, NS58D and NS41D. Passive protective activities in rabbit antisera prepared with strains Smith, NS58D and NS41D were absorbed out with either homologous cell surface polysaccharide fraction or cell surface polysaccharide fraction extracted from strain ST67P. Well-defined large capsules were observed in ultra-thin sections treated with rabbit antiserum prepared with homologous strain conjugated with ferritin. Also, the capsule surrounded by ferritin granules was shown in ultra-thin sections treated with ferritin conjugated with antisera prepared with those heterologous strains although the capsular size was significantly smaller than those observed by homologous antiserum.
猪葡萄球菌ST67P菌株在普通血清软琼脂中呈弥漫性生长。分别加入用A型、B型或C型金黄色葡萄球菌菌株（史密斯株、NS58D株或NS41D株）制备的兔抗血清后，该菌转变为致密型生长。用ST67P菌株的热灭活疫苗免疫的小鼠对同源或异源菌株（史密斯株、NS58D株和NS41D株）均表现出显著抗性。用史密斯株、NS58D株和NS41D株制备的兔抗血清中的被动保护活性，可被同源细胞表面多糖组分或从ST67P菌株中提取的细胞表面多糖组分吸收。在用与铁蛋白偶联的同源菌株制备的兔抗血清处理的超薄切片中，观察到界限分明的大荚膜。此外，在用与那些异源菌株制备的抗血清偶联的铁蛋白处理的超薄切片中，也显示出被铁蛋白颗粒包围的荚膜，尽管荚膜大小明显小于用同源抗血清观察到的荚膜。
