WILEY B B, WONNACOTT J C
J Bacteriol. 1962 Jun;83(6):1169-76. doi: 10.1128/jb.83.6.1169-1176.1962.
Wiley, Bill B. (University of Saskatchewan, Saskatoon, Sask., Canada) and Jane C. Wonnacott. Isolation and partial characterization of a capsular material from Staphylococcus aureus. J. Bacteriol. 83:1169-1176. 1962.-An encapsulated strain of Staphylococcus aureus, which underwent a specific capsular reaction with homologous antiserum, was grown in a semisynthetic Casamino acids-glycerol broth. A fraction containing the capsular material was isolated from the culture supernatants. The preparation was antigenic in rabbits and absorbed the antibody responsible for both the passive protection of chick embryos and the specific capsular reaction in rabbit antiserum against the homologous organism. Chemically, the material was free of nucleic acids, contained 8% nitrogen, 4.25% total phosphorus, 26% hexosamine, and 26% reducing sugar. Acid hydrolysis yielded the amino acids glycine, alanine, glutamic acid, and lysine. Glucosamine and organic phosphate compounds were also present. Organic phosphate compounds were accounted for as glycerophosphate.
威利，比尔·B.（加拿大萨斯喀彻温省萨斯卡通市萨斯喀彻温大学）和简·C.·沃纳科特。金黄色葡萄球菌荚膜物质的分离及部分特性研究。《细菌学杂志》83:1169 - 1176。1962年。——一株能与同源抗血清发生特异性荚膜反应的金黄色葡萄球菌在半合成酪蛋白氨基酸 - 甘油肉汤中培养。从培养上清液中分离出含有荚膜物质的部分。该制剂在兔体内具有抗原性，能吸收负责鸡胚被动保护以及兔抗血清中针对同源菌的特异性荚膜反应的抗体。从化学组成来看，该物质不含核酸，含氮量为8%，总磷量为4.25%，己糖胺为26%，还原糖为26%。酸水解产生甘氨酸、丙氨酸、谷氨酸和赖氨酸。还存在葡萄糖胺和有机磷酸盐化合物。有机磷酸盐化合物以甘油磷酸的形式存在。
