大肠杆菌中所有稳定RNA种类通过转录后修饰形成。
Formation of all stable RNA species in Escherichia coli by posttranscriptional modification.
作者信息
Pace B, Peterson R L, Pace N R
出版信息
Proc Natl Acad Sci U S A. 1970 Apr;65(4):1097-104. doi: 10.1073/pnas.65.4.1097.
Abstract
The kinetics of accumulation of the known stable RNA species (23S, 16S, and 5S rRNA and tRNA) in Escherichia coli C122 were monitored by polyacrylamide gel electrophoresis of purified cellular RNA, following termination of brief pulse labeling with (32)P-orthophosphate. Isotopically labeled stable RNA species appear only after a time lag, while total cellular RNA and the ostensible precursors to the stable RNA classes accumulate from the earliest times examined. It is concluded that all the known stable RNA species in E. coli are the products of posttranscriptional modification.
摘要
用（32）P-正磷酸盐进行短暂脉冲标记后，通过对纯化的细胞RNA进行聚丙烯酰胺凝胶电泳，监测了大肠杆菌C122中已知稳定RNA种类（23S、16S和5S rRNA以及tRNA）的积累动力学。同位素标记的稳定RNA种类仅在一段时间延迟后出现，而总细胞RNA和稳定RNA类别的表面前体从最早检测的时间就开始积累。得出的结论是，大肠杆菌中所有已知的稳定RNA种类都是转录后修饰的产物。
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本文引用的文献
1
[Apropos of the presence of weak molecular weight RNA in the ribosomes of Escherichia Coli].[关于大肠杆菌核糖体中低分子量RNA的存在]
Biochim Biophys Acta. 1963 Apr 30;68:653-6. doi: 10.1016/0006-3002(63)90199-9.
2
The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.用聚丙烯酰胺凝胶电泳法分离高分子量核糖核酸
Biochem J. 1967 Jan;102(1):251-7. doi: 10.1042/bj1020251.
3
Electrophoretic separation of viral nucleic acids on polyacrylamide gels.病毒核酸在聚丙烯酰胺凝胶上的电泳分离。
J Mol Biol. 1967 Jun 28;26(3):373-87. doi: 10.1016/0022-2836(67)90310-5.
4
Biosynthesis of ribosomes in bacterial cells.细菌细胞中核糖体的生物合成。
Prog Nucleic Acid Res Mol Biol. 1965;4:161-88. doi: 10.1016/s0079-6603(08)60787-4.
5
Specificity in the structure of transfer RNA.转运RNA结构的特异性
Prog Nucleic Acid Res Mol Biol. 1967;6:39-82. doi: 10.1016/s0079-6603(08)60524-3.
6
Some properties of ribonucleic acid obtained from ribosomal precursors of Escherichia coli.从大肠杆菌核糖体前体中获得的核糖核酸的一些性质。
Biochim Biophys Acta. 1965 Jun 8;103(2):241-51. doi: 10.1016/0005-2787(65)90165-6.
7
The kinetics of product appearance and template involvement in the in vitro replication of viral RNA.病毒RNA体外复制过程中产物出现的动力学及模板参与情况。
Proc Natl Acad Sci U S A. 1967 Aug;58(2):711-8. doi: 10.1073/pnas.58.2.711.
8
An extracellular Darwinian experiment with a self-duplicating nucleic acid molecule.一项针对自我复制核酸分子的细胞外达尔文实验。
Proc Natl Acad Sci U S A. 1967 Jul;58(1):217-24. doi: 10.1073/pnas.58.1.217.
9
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J Bacteriol. 1969 Aug;99(2):535-43. doi: 10.1128/jb.99.2.535-543.1969.
10
"Transcriptional mapping". I. Introduction to the method and the use of actinomycin D as a transcriptional mapping agent.“转录图谱分析”。一、该方法简介以及放线菌素D作为转录图谱分析试剂的应用
Proc Natl Acad Sci U S A. 1969 Jun;63(2):532-9. doi: 10.1073/pnas.63.2.532.
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