“冠状病毒”在组织培养中的传播。
The propagation of "coronaviruses" in tissue-culture.
作者信息
Bradburne A F, Tyrrell D A
出版信息
Arch Gesamte Virusforsch. 1969;28(2):133-50. doi: 10.1007/BF01249379.
Abstract
The conditions for the cultivation of the 229-E respiratory virus in tissue culture are described. The virus multiplies only in human tissue culture systems, but a variety of cell types are sensitive to it. The virus has been isolated in three continuous cell lines and a plaque assay has been developed in one of these. This cell line (L 132) derived from human embryo lung, was also used to isolate three other viruses of similar morphology which had previously been cultivated only in organ cultures of human respiratory epithelium. High rates of isolation of these viruses were made in L 132 cells using nasal washings from experimentally inoculated volunteers. It is concluded that this cell line is a valuable tool in the study of viruses of this group.
摘要
本文描述了229-E呼吸道病毒在组织培养中的培养条件。该病毒仅在人组织培养系统中增殖，但多种细胞类型对其敏感。该病毒已在三种连续细胞系中分离出来，并在其中一种细胞系中建立了空斑试验。这种源自人胚肺的细胞系（L 132）也被用于分离其他三种形态相似的病毒，这些病毒以前仅在人呼吸道上皮器官培养物中培养。使用来自实验接种志愿者的鼻洗液，在L 132细胞中高频率地分离出这些病毒。结论是，该细胞系是研究这类病毒的宝贵工具。
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