Harrison R J
J Clin Pathol. 1970 Apr;23(3):219-24. doi: 10.1136/jcp.23.3.219.
A reliable method is described for the assay of vitamin B(12) activity in erythrocytes using Lactobacillus leichmannii. The results are shown for age, sex, and blood group in normal subjects, and before, during, and after therapy in vitamin B(12) deficiency anaemia. The normal range is 85-225 pg/ml (mean = 155 +/- 35 pg/ml). Raised levels were found in the newborn, and subnormal levels in subjects aged 70 years and over and in males aged 10 to 19 years. There were no differences in erythrocyte vitamin B(12) levels between the ABO or Rhesus blood groups. In pernicious anaemia low levels are found before treatment (mean = 91 pg/ml), followed by a rapid rise during vitamin B(12) therapy (mean peak = 482 pg/ml), and falling more slowly towards normal after the initial haematological response.
描述了一种使用赖氏乳杆菌测定红细胞中维生素B12活性的可靠方法。给出了正常受试者的年龄、性别和血型的结果，以及维生素B12缺乏性贫血患者治疗前、治疗期间和治疗后的结果。正常范围为85 - 225 pg/ml（平均值 = 155 ± 35 pg/ml）。新生儿的水平升高，70岁及以上的受试者以及10至19岁的男性水平低于正常。ABO血型或恒河猴血型之间的红细胞维生素B12水平没有差异。在恶性贫血中，治疗前水平较低（平均值 = 91 pg/ml），维生素B12治疗期间迅速上升（平均峰值 = 482 pg/ml），在最初的血液学反应后向正常水平下降得更慢。
