Rosen B, Christiansen L N, Busta F F
Appl Microbiol. 1970 Jul;20(1):113-6. doi: 10.1128/am.20.1.113-116.1970.
Heated combinations of trimethylamine oxide (TMAO) and culture media (tryptone, glucose, yeast extract broth or a defined minimal medium), or heated TMAO and glucose, contained substance(s) that inhibited growth of Bacillus megaterium. Inhibition was expressed primarily as an increase of the lag phase of growth; the logarithmic growth rate was comparable to control cultures. The addition of unheated TMAO to the culture media had no effect on growth. Results suggested that TMAO was decomposed during heating and that dimethylamine, one of the degradation products, reacted with glucose by a Maillard-Amadori reaction to produce the inhibitory substance(s).
氧化三甲胺（TMAO）与培养基（胰蛋白胨、葡萄糖、酵母提取物肉汤或特定的基本培养基）的加热组合，或加热的TMAO与葡萄糖，含有抑制巨大芽孢杆菌生长的物质。抑制作用主要表现为生长延迟期延长；对数生长速率与对照培养物相当。向培养基中添加未加热的TMAO对生长没有影响。结果表明，TMAO在加热过程中分解，降解产物之一二甲胺通过美拉德-阿马多里反应与葡萄糖反应生成抑制性物质。
