Forsén N R, Alspaugh M, Barnett E V
Immunology. 1970 Oct;19(4):669-76.
(1) Antibodies reactive with purified DNA in the complement fixation system and induced by active immunization of rabbits with methylated bovine serum albumin—DNA aggregate (MBSA—DNA) have been previously described. The present study demonstrates their reactivity in the quantitative and double diffusion preciptin systems. In these systems they have shown preferential reactivity with heat denatured DNA and great cross-reactivity with denatured DNA from various sources. (2) Methylated rabbit serum albumin—DNA aggregate (MRSA—DNA) is an effective immunizing antigen resulting in the production of antibodies similar to those produced by MBSA—DNA. (3) The precipitating antibodies in this system are 19S immunoglobulins and appear to be identical to the complement fixing antibodies. (4) The precipitin reaction is not significantly inhibited by mono-nucleotides.
(1) 先前已描述过,通过用甲基化牛血清白蛋白 - DNA 聚集体(MBSA - DNA)对兔子进行主动免疫诱导产生的、在补体结合系统中与纯化 DNA 反应的抗体。本研究证明了它们在定量和双向扩散沉淀素系统中的反应性。在这些系统中,它们对热变性 DNA 表现出优先反应性,并且与来自各种来源的变性 DNA 具有很强的交叉反应性。(2) 甲基化兔血清白蛋白 - DNA 聚集体(MRSA - DNA)是一种有效的免疫抗原,可产生与 MBSA - DNA 产生的抗体相似的抗体。(3) 该系统中的沉淀抗体是 19S 免疫球蛋白,似乎与补体结合抗体相同。(4) 沉淀素反应不受单核苷酸的显著抑制。