Jayanetra P, Vorachit M, Bhatarakamol S
Southeast Asian J Trop Med Public Health. 1975 Mar;6(1):10-7.
The cultural, biochemical characteristics and antibiotic sensitivity of strains of Pseudomonas pseudomallei isolated from four cases of melioidosis admitted to Ramathibodi Hospital are described. The organisms were gram-negative bacilli often with bipolar staining. The colonies were wrinkled when incubated for long periods. The characteristic non-specific uptake of dye from media into the colonies and their musty or earthy odour rendered them easily distinguishable from other organisms. All strains were sensitive to chloramphenicol and all but one were sensitive to tetracycline. All strains were resistant to colimycin and gentamicin. The pathogenicity of the strain isolated from a fatal case of peritonitis was studied in guinea pigs. The findings showed that following a large inoculation intraperitoneally, the animal developed acute septicaemia and died shortly afterwards. Only a few micro-abscesses were found on the surface of the liver. Chronic infection of longer duration occurred when a small number of organisms were introduced through a cutaneous abrasion. The lesions included pneumonitis and multiple abscesses of various organs including subcutaneous tissue, liver, spleen and mediastinum.
本文描述了从拉玛蒂博迪医院收治的4例类鼻疽患者中分离出的类鼻疽杆菌菌株的培养特性、生化特性及抗生素敏感性。这些菌株为革兰氏阴性杆菌，常呈两极染色。长时间培养后菌落呈皱缩状。其具有从培养基非特异性摄取染料进入菌落的特性以及发霉或土腥味，这使得它们易于与其他微生物区分开来。所有菌株对氯霉素敏感，除1株外对四环素均敏感。所有菌株对黏菌素和庆大霉素耐药。对从1例致命性腹膜炎病例中分离出的菌株在豚鼠身上进行了致病性研究。结果显示，经腹腔大量接种后，动物发生急性败血症并很快死亡。肝脏表面仅发现少量微脓肿。通过皮肤擦伤接种少量菌株时会发生持续时间较长的慢性感染。病变包括肺炎以及包括皮下组织、肝脏、脾脏和纵隔在内的各器官的多发性脓肿。
