Pathological changes in goats experimentally inoculated with Pseudomonas pseudomallei.

Sixteen goats were inoculated subcutaneously or intraperitoneally with cultured Pseudomonas pseudomallei isolated from a goat. They showed either acute or chronic phase of disease. When inoculated intraperitoneally, animals suffered from a septicemic illness of short duration accompanied with micro-abscesses scattered widely throughout the body. In animals subcutaneously inoculated no clinical signs were exhibited, but the disease was of rather long duration, and lesions were large and localized in the spleen and lungs. Coincident with lesions, a large number of Pseudomonas pseudomallei organisms were isolated from abscesses in the spleen and lungs. Ultrastructurally, numerous bacilli could be detected in the cytoplasm of macrophages in the abscesses. They had an electron-dense cell wall. Most of them were surrounded by electron-dense substance.