体细胞杂种的血清蛋白合成

Serum protein synthesis by somatic cell hybrids.

作者信息

Wray L K, Sutton H E

出版信息

Somatic Cell Genet. 1982 Jul;8(4):433-49. doi: 10.1007/BF01538706.

Abstract

Serum protein synthesis by rodent hepatomaa x fibroblast, hepatom a x teratocarcinoma, and hepatoma x hepatoma somatic cell hybrids was analyzed by Laurell (rocket) and crossed immunoelectrophoresis. With the hepatoma x fibroblast hybrids, of the nine serum proteins investigated, only transferrin was synthesized by the hybrids (with the exception of one hybrid clone which made albumin). Rat hepatoma (MHC) x mouse teratocarcinoma hybrids did not synthesize transferrin, but one of three clones did produce albumin. Rat hepatopma (Faza) x mouse hepatoma (Hepa 1) hybrids synthesized reduced amounts of albumin and transferrin, compared with the parental hepatoma cell lines. From these studies transferrin synthesis appears to be under different controls from the other serum proteins examined.

摘要

通过劳雷尔（火箭）免疫电泳和交叉免疫电泳分析了啮齿动物肝癌细胞与成纤维细胞、肝癌细胞与畸胎瘤细胞以及肝癌细胞与肝癌细胞体细胞杂种的血清蛋白合成情况。在肝癌细胞与成纤维细胞杂种中，在所研究的9种血清蛋白中，杂种细胞仅合成转铁蛋白（有一个杂种克隆合成白蛋白的情况除外）。大鼠肝癌（MHC）×小鼠畸胎瘤杂种细胞不合成转铁蛋白，但三个克隆中有一个确实产生白蛋白。与亲代肝癌细胞系相比，大鼠肝癌（法扎）×小鼠肝癌（Hepa 1）杂种细胞合成的白蛋白和转铁蛋白量减少。从这些研究来看，转铁蛋白的合成似乎受与所检测的其他血清蛋白不同的调控。