Shimizu N, Sokawa Y
J Biol Chem. 1979 Dec 10;254(23):12034-7.
The activity of 2',5'-oligoadenylate synthetase, an enzyme recently discovered in interferon-treated cells, was found in lymphocytes from normal mouse spleen that had received neither exogenous interferon nor its inducers. The oligoadenylate synthesized by lymphocyte cell extracts inhibited protein synthesis in rabbit reticulocyte lysates. The oligomers were composed mainly of trimer and were resistant to digestion by T2 ribonuclease. The level of the enzyme in lymphocytes was about 20 to 30% of that in L929 cells treated with interferon. The activity of the enzyme was further enhanced in lymphocytes in vitro by addition of interferon. The 2',5'-oligoadenylate synthetase was distributed among several lymphoid tissues, but was not detected in cell extracts from brain or liver. The enzyme may play an important role in the regulation of the immune system.
2',5'-寡腺苷酸合成酶是一种最近在经干扰素处理的细胞中发现的酶，在未接受外源性干扰素及其诱导剂的正常小鼠脾脏淋巴细胞中也被发现。淋巴细胞提取物合成的寡腺苷酸可抑制兔网织红细胞裂解物中的蛋白质合成。这些寡聚物主要由三聚体组成，对T2核糖核酸酶的消化具有抗性。淋巴细胞中该酶的水平约为经干扰素处理的L929细胞中的20%至30%。通过添加干扰素，该酶在体外淋巴细胞中的活性进一步增强。2',5'-寡腺苷酸合成酶分布于多种淋巴组织中，但在脑或肝的细胞提取物中未检测到。该酶可能在免疫系统的调节中发挥重要作用。
