用于测定少量碳氧血红蛋白的不同分光光度法的比较评估

Comparative evaluation of different spectrophometric methods for the determination of small amounts of carboxyhaemoglobin.

作者信息

Heinemann G, Löschenkohl K, Schievelbein H

出版信息

J Clin Chem Clin Biochem. 1979 Oct;17(10):647-51. doi: 10.1515/cclm.1979.17.10.647.

DOI:10.1515/cclm.1979.17.10.647

Abstract

Four spectrophotometric methods measuring HbCO were investigated in comparison with an analyzer (CO-Oximeter) in order to find a method determining HbCO fractions) below 0.10 with sufficient reliability. The CO-Oximeter was chosen as "reference" method because results of other authors with this instrument yielded good agreement with other methods, e.g. IR-spectrometry, GLC, van Slyke. Only the five wavelength method of Commins & Lawther (1965) Brit. J. Industr. Med. 22, 139--143) can be recommended, for it yielded CV's and sensitivities comparable to the CO-Oximeter. The within-run precision had CVs of 2.3% to 4.7%, the sensitivity was 0.0021 to 0.0028. The method is suited even for HbCO measurements of shorter series.

摘要

为了找到一种能可靠测定低于0.10的碳氧血红蛋白（HbCO）含量的方法，我们研究了四种分光光度法测定HbCO，并与一种分析仪（一氧化碳血氧计）进行了比较。一氧化碳血氧计被选为“参考”方法，因为其他作者使用该仪器得到的结果与其他方法（如红外光谱法、气相色谱法、范斯莱克法）取得了良好的一致性。只有康明斯和劳瑟（1965年，《英国工业医学杂志》22卷，139 - 143页）的五波长法值得推荐，因为它的变异系数（CV）和灵敏度与一氧化碳血氧计相当。批内精密度的CV为2.3%至4.7%，灵敏度为0.0021至0.0028。该方法甚至适用于较短系列的HbCO测量。