Katsumata Y, Aoki M, Sato K, Suzuki O, Oya M, Yada S
J Forensic Sci. 1982 Oct;27(4):928-34.
A simple spectrophotometric method for determination of carboxyhemoglobin (HbCO) in blood is described. Blood is dissolved in sodium carbonate solution at a dilution near 250-fold. The diluent contains sodium hydrosulfite providing the two-component system HbCO- reduced hemoglobin (HHb). The percentage of HbCO is calculated from absorbance values at 532 and 558 nm measured after the addition of sodium hydroxide, which makes the solution completely clear. Results obtained by the present method are in satisfactory agreement with those by the oxygen electrode method.
本文描述了一种用于测定血液中碳氧血红蛋白（HbCO）的简单分光光度法。将血液溶解在碳酸钠溶液中，稀释倍数接近250倍。稀释剂中含有连二亚硫酸钠，形成HbCO-还原血红蛋白（HHb）两组分体系。加入氢氧化钠使溶液完全澄清后，根据在532和558nm处测得的吸光度值计算HbCO的百分比。本方法得到的结果与氧电极法的结果吻合良好。
