一种测定碳氧血红蛋白的新方法。
A new method of carboxyhaemoglobin determination.
作者信息
Sanderson J H, Sotheran M F, Stattersfield J P
出版信息
Br J Ind Med. 1978 Feb;35(1):67-72. doi: 10.1136/oem.35.1.67.
Abstract
A quick and accurate method of determining the concentration of carboxyhaemoglobin (COHb) in blood has been developed. The method uses a dual wavelength double beam spectrophotometer in the 1st derivative mode, linked to a digital voltmeter (DVM), with the two beams set 3 nm apart around an isobestic point of reduced haemoglobin (Hbred) and carboxyhaemoglobin at 579 nm. The 1st derivative mode measures the slope, and this slope is proportional to the concentration of COHb.
摘要
已开发出一种快速准确测定血液中碳氧血红蛋白（COHb）浓度的方法。该方法采用处于一阶导数模式的双波长双光束分光光度计，连接到数字电压表（DVM），两束光在还原血红蛋白（Hbred）和碳氧血红蛋白在579nm处的等吸收点周围设置为相距3nm。一阶导数模式测量斜率，该斜率与COHb的浓度成正比。
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本文引用的文献
1
CARBON MONOXIDE IN BLOOD: ANALYTICAL METHOD AND SOURCES OF ERROR.血液中的一氧化碳：分析方法与误差来源
J Appl Physiol. 1964 May;19:510-5. doi: 10.1152/jappl.1964.19.3.510.
2
A rapid method for simultaneous measurement of carboxy- and methemoglobin in blood.一种同时测定血液中碳氧血红蛋白和高铁血红蛋白的快速方法。
J Appl Physiol. 1971 Jul;31(1):154-60. doi: 10.1152/jappl.1971.31.1.154.
3
An evaluation of the spectrophotometric determination of HbO2, and Hb in blood with the co-oximeter IL 182.使用IL 182型血氧计对血液中氧合血红蛋白（HbO2）和血红蛋白（Hb）的分光光度测定法进行评估。
Clin Chim Acta. 1970 Aug;29(2):303-9. doi: 10.1016/0009-8981(70)90051-3.
4
The determination of carbon monoxide in blood and tissue.血液和组织中一氧化碳的测定。
Analyst. 1970 May;95(130):439-58. doi: 10.1039/an9709500439.
5
Determination of carbon monoxide in blood.
Anal Chem. 1972 Jul;44(8):1529-30. doi: 10.1021/ac60316a060.
6
New method for rapid determination of carboxyhemoglobin by use of double-wavelength spectrophotometry.利用双波长分光光度法快速测定碳氧血红蛋白的新方法。
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7
Hemoglobin pigments. Spectrophotometric determination of oxy-, carboxy-, met-, and sulfhemoglobin in capillary blood.血红蛋白色素。分光光度法测定毛细血管血中的氧合血红蛋白、碳氧血红蛋白、高铁血红蛋白和硫化血红蛋白。
Clin Chim Acta. 1972 Nov;42(1):85-100. doi: 10.1016/0009-8981(72)90380-4.
8
Hemoglobin pigments. Photometer for oxygen saturation, carboxyhemoglobin, and methemoglobin in capillary blood.血红蛋白色素。用于检测毛细血管血中氧饱和度、碳氧血红蛋白和高铁血红蛋白的光度计。
Clin Chim Acta. 1972 Nov;42(1):101-8. doi: 10.1016/0009-8981(72)90381-6.
9
Spectrophotometric measurement of carboxyhaemoglobin. An evaluation of the method of Commins and Lawther.羧基血红蛋白的分光光度测量。对康明斯和劳瑟方法的评估。
Br J Ind Med. 1972 Oct;29(4):454-7. doi: 10.1136/oem.29.4.454.
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