羧基血红蛋白。采用一种测量血液中一氧化碳的新参考方法进行测试和校准的分光光度法测定。

Carboxyhaemoglobin. Spectrophotometric determination tested and calibrated using a new reference method for measuring carbon monoxide in blood.

作者信息

Dijkhuizen P, Buursma A, Gerding A M, Van Kampen E J, Zijlstra W G

出版信息

Clin Chim Acta. 1977 Oct 1;80(1):95-104. doi: 10.1016/0009-8981(77)90268-6.

Abstract

The spectrophotometric determination of HbCO at lambda = 562 and 540 nm in the system HbCO/HbO2 was reinvestigated using a new reference method for measuring CO in blood. This reference method is based on the conversion of CO from HbCO into CO2 which is determined by titration. Plotting the absorbance ratio A562/A540 against the titrimetrically determined HbCO fractions of 46 blood samples demonstrated a linear relationship up to 90% HbCO and yielded more accurate values for the constants in the equation for calculating the HbCO fraction from A562/A540. The standard deviation of the differences between the spectrophotometric and the titrimetric method was 1.2% HbCO. It is shown that the influence of other haemoglobin derivatives and other possible sources of error is either negligible or can be prevented by simple precautions.

摘要

采用一种测定血液中一氧化碳（CO）的新参考方法，对在波长λ = 562和540 nm下分光光度法测定HbCO/HbO₂体系中的HbCO进行了重新研究。该参考方法基于将HbCO中的CO转化为通过滴定法测定的CO₂。将46份血样的吸光度比值A562/A540与滴定法测定的HbCO分数作图，结果表明，在高达90% HbCO的范围内呈线性关系，并且从A562/A540计算HbCO分数的方程中的常数得出了更准确的值。分光光度法和滴定法之间差异的标准差为1.2% HbCO。结果表明，其他血红蛋白衍生物的影响以及其他可能的误差来源要么可以忽略不计，要么可以通过简单的预防措施加以避免。