使用薄层色谱法对非发酵革兰氏阴性菌进行核酸酶研究。
Nucleic acid enzyme studies of nonfermentative gram-negative bacteria using thin-layer chromatography.
作者信息
Klein M M, Blazevic D J
出版信息
Appl Microbiol. 1972 Feb;23(2):276-80. doi: 10.1128/am.23.2.276-280.1972.
A rapid and easy to perform procedure for determining the nucleic acid enzyme reactions of intact bacterial cells was developed. Overnight organism growth on triple sugar agar was tested for nucleoside phosphotransferase, nucleosidase, and nucleotidase activity. Reaction products were detected by means of thin-layer chromatography and fluorescence. Characteristic patterns were seen with certain strains of nonfermentative gram-negative bacteria, which indicated that these tests could aid in classification and identification of E0-1 group, Pseudomonas multivorans, P. cepacia, P. maltophilia, certain other Pseudomonas species, and Herellea vaginicola.
开发了一种快速且易于执行的程序，用于确定完整细菌细胞的核酸酶反应。检测了在三糖琼脂上过夜培养的生物体中核苷磷酸转移酶、核苷酶和核苷酸酶的活性。通过薄层色谱法和荧光检测反应产物。在某些非发酵革兰氏阴性菌菌株中观察到特征性图谱，这表明这些测试有助于对E0-1组、多食假单胞菌、洋葱伯克霍尔德菌、嗜麦芽窄食单胞菌、某些其他假单胞菌属物种以及阴道海雷菌进行分类和鉴定。
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