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噬菌体T4含胞嘧啶DNA经序列特异性内切酶SalI和KpnI的切割图谱。

Cleavage map of bacteriophage T4 cytosine-containing DNA by sequence-specific endonucleases SalI and KpnI.

作者信息

Carlson K, Nicolaisen B

出版信息

J Virol. 1979 Jul;31(1):112-23. doi: 10.1128/JVI.31.1.112-123.1979.

DOI:10.1128/JVI.31.1.112-123.1979
PMID:501794
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC353427/
Abstract

Cytosine-containing T4 DNA from endoII- endoIV- dCTPase- alc2 phage grown in a sup+ rB- mB- host is cleaved by endo R.EcoRI and endo R.HindIII to greater than 40 fragments and by endo R.SalI and endo R.KpnI to 8 and 6 fragments, respectively. The latter two fragment sets have been correlated to each other to produce a cleavage map of the genome. The sum of the molecular weights of the fragments calculated from electrophoretic mobility in agarose gels yields a genome molecular weight for cytosine-containing T4 DNA of 105 x 10(6).

摘要

在sup+ rB- mB-宿主中生长的endoII- endoIV- dCTPase- alc2噬菌体的含胞嘧啶T4 DNA，被内切酶R.EcoRI和内切酶R.HindIII切割成40多个片段，被内切酶R.SalI和内切酶R.KpnI分别切割成8个和6个片段。后两组片段已相互关联以产生基因组的切割图谱。根据琼脂糖凝胶中的电泳迁移率计算出的片段分子量总和得出含胞嘧啶T4 DNA的基因组分子量为105×10(6)。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa2f/353427/cb5e525ee4c8/jvirol00187-0125-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa2f/353427/f0fedebf36a9/jvirol00187-0122-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa2f/353427/cb5e525ee4c8/jvirol00187-0125-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa2f/353427/f0fedebf36a9/jvirol00187-0122-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa2f/353427/cb5e525ee4c8/jvirol00187-0125-a.jpg

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本文引用的文献

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Map of restriction sites on bacteriophage T4 cytosine-containing DNA for endonucleases bamHI, BglII, KpnI, PvuI, SalI, and XbaI.噬菌体T4含胞嘧啶DNA上针对核酸内切酶BamHI、BglII、KpnI、PvuI、SalI和XbaI的限制酶切位点图谱。
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