Bossen E H, Rowlands D T
Immunology. 1972 Mar;22(3):417-21.
Buoyant density centrifugation of linear albumin gradients was used to fractionate suspensions of guinea-pig spleen cells. The fractions obtained were studied with respect to morphology and function of their cells. Using this technique it was shown that cells responsible for the production of sheep cell haemolysin were located in fractions distinct from those which could be used for the passive transfer of delayed tuberculoprotein hypersensitivity.
采用线性白蛋白梯度的浮力密度离心法对豚鼠脾细胞悬液进行分级分离。对所获得的各组分的细胞形态和功能进行了研究。利用该技术表明,负责产生绵羊细胞溶血素的细胞位于与可用于被动转移迟发型结核蛋白超敏反应的细胞不同的组分中。