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采用噬斑抑制技术在细胞水平测定抗体亲和力：抑制剂价数的影响

Determination of antibody avidity at the cellular level by the plaque inhibition technique: effect of valence of the inhibitor.

作者信息

Goidl E A, Birnbaum G, Siskind G W

出版信息

J Immunol Methods. 1975;8(1-2):47-5. doi: 10.1016/0022-1759(75)90080-0.

DOI:10.1016/0022-1759(75)90080-0
PMID:50381
Abstract

Inhibition of plaque formation by multivalent and univalent ligands was compared as an assay of avidity of antibody produced by PFC. Multivalent ligands are much more effective as inhibitors and their use tends to impart an appearance of lack of heterogeneity and high avidity to the PFC populations being studied. It is thus probably generally advisable to employ univalent ligands in such studies.

摘要

比较多价和单价配体对斑块形成的抑制作用，以此作为对由空斑形成细胞（PFC）产生的抗体亲和力的一种测定方法。多价配体作为抑制剂更为有效，并且使用多价配体往往会使所研究的PFC群体呈现出缺乏异质性和高亲和力的表象。因此，在这类研究中使用单价配体可能通常是明智的。

相似文献

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Determination of antibody avidity at the cellular level by the plaque inhibition technique: effect of valence of the inhibitor.采用噬斑抑制技术在细胞水平测定抗体亲和力：抑制剂价数的影响
J Immunol Methods. 1975;8(1-2):47-5. doi: 10.1016/0022-1759(75)90080-0.
2
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Rosette-plaque assay: fine specificity differences among individual hapten-primed lymph node cells.玫瑰花结-噬菌斑试验：单个半抗原致敏淋巴结细胞之间的精细特异性差异。
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Analysis of the temporal changes in the avidity of primary IgM and IgG antibody at the cellular level.在细胞水平分析原发性IgM和IgG抗体亲和力的时间变化。
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Studies on the regulation of avidity at the level of the single antibody-forming cell. The effect of antigen dose and time after immunization.关于单个抗体形成细胞水平亲和力调节的研究。抗原剂量和免疫后时间的影响。
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Antigenic selection of IgG precursor subpopulations.IgG前体亚群的抗原选择
J Immunol. 1975 Aug;115(2):495-501.

引用本文的文献

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Infect Immun. 1990 May;58(5):1261-8. doi: 10.1128/iai.58.5.1261-1268.1990.
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Ontogeny of B-lymphocyte function. II. Ability of endotoxin to increase the heterogeneity of affinity of the immune response of B lymphocytes from fetal mice.B淋巴细胞功能的个体发生。II. 内毒素增加胎鼠B淋巴细胞免疫反应亲和力异质性的能力。
J Exp Med. 1976 Jun 1;143(6):1503-20. doi: 10.1084/jem.143.6.1503.
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Studies on the control of antibody synthesis. XIII. Preferential depeletion of precursors of high affinity antibody-secreting cells by specific immunoadsorbents.
抗体合成控制的研究。十三。特异性免疫吸附剂对高亲和力抗体分泌细胞前体的优先耗竭。
Immunology. 1979 Sep;38(1):145-56.
4
Detection and analysis of recognition and selection in the immune response.免疫反应中识别与选择的检测及分析
Bull Math Biol. 1977;39(6):705-19. doi: 10.1007/BF02461779.
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Production of auto-anti-idiotypic antibody during the normal immune response to TNP-ficoll. I. Occurrence in AKR/J and BALB/c mice of hapten-augmentable, anti-TNP plaque-forming cells and their accelerated appearance in recipients of immune spleen cells.在对三硝基苯-聚蔗糖（TNP-ficoll）的正常免疫反应过程中自身抗独特型抗体的产生。I. AKR/J和BALB/c小鼠中半抗原增强的抗TNP噬斑形成细胞的出现及其在免疫脾细胞受体中的加速出现。
J Exp Med. 1979 Jul 1;150(1):138-53. doi: 10.1084/jem.150.1.138.
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Ontogeny of B-lymphocyte function. III. In vivo and in vitro studies on the ease of tolerance induction in B lymphocytes from fetal, neonatal, and adult mice.B淋巴细胞功能的个体发生。III. 关于胎儿、新生和成年小鼠B淋巴细胞诱导耐受性难易程度的体内和体外研究。
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Ontogeny of B-lymphocyte function. V. Thymus cell involvement in the functional maturation of B-lymphocytes from fetal mice transferred into adult irradiated hosts.B淋巴细胞功能的个体发生。V. 胸腺细胞参与转入成年受辐照宿主的胎鼠B淋巴细胞的功能成熟过程。
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Differences in the mechanism of tolerance to dinitrophenylated bovine gamma globulin when induced in normal adult mice or in reconstituted irradiated mice: dependence of the mechanism of tolerance on the structural organization of the lymphoid system.正常成年小鼠或经辐射后重建的小鼠诱导产生对二硝基苯基化牛γ球蛋白耐受性的机制差异：耐受性机制对淋巴系统结构组织的依赖性。
J Exp Med. 1977 Apr 1;145(4):968-82. doi: 10.1084/jem.145.4.968.